Erythropoiesis-inhibiting factor(s) (EIF): methodologic studies

Purification and characterization of protein synthesis initiation factors eIF-1, eIF-4C, eIF-4D, and eIF-5 from rabbit reticulocytes.

Protein synthesis initiation factors eIF-1, eIF-4C, eIF4D, and eIF-5 were isolated and purified from rabbit reticulocyte lysates. Crude initiation factors from the high salt ribosomal extract were separated by ammonium sulfate precipitation into Fraction A (0 to 40% saturation) and Fraction BC (40 to 70% saturation). Fraction BC was separated by gel filtration on Sephadex G-100 into two fractio...

MSH-release inhibiting factors: recent studies.

The evidence which has finally resulted in the general acceptance of the concept that there are substances present in hypothalamic tissue which inhibit the release of melanocyte-stimulating hormone (MSH) from the pituitary glands of mammals as well as amphibians has been summarized in several recent reviews (1-3). Although the chemical structures of several compounds with such activity are know...

Studies on erythropoiesis.

Studies on the erythropoiesis inhibiting factor in the plasma of animals with transfusion polycythemia.

I T HAS BEEN proved by many authors that polycythemia induced by blood transfusion reduces the peripheral reticulocyte count and inhibits erythropoiesis. #{176} In previous investigations on erythropoiesis We have observed that polycythemia causes the appearance in the plasma of an active substance, which is antagonistic to erythropoietin. The plasma obtained from polycythemic animals was injec...

Methodologic issues in injury case-control studies.

In this paper some methodological problems particularly relevant to case-control studies of injury are illustrated by reference to previous childhood injury case-control studies. In contrast to studies of disease, where 'person time' constitutes the observational experience of interest, in injury studies person time engaged in a particular activity to often more appropriate. The implications fo...